| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.044543 |
| Chromosome: | chromosome 16 |
| Location: | 1867993 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g655600 | (1 of 4) PF16016 - Domain of unknown function (DUF4782) (DUF4782) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCTGGCTCGTTGCGGAGTGGAATCTCCAT |
| Internal bar code: | TGGGAATCGAGAATCGTGTATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 196 |
| LEAP-Seq percent confirming: | 99.7344 |
| LEAP-Seq n confirming: | 751 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAGTCCATGCCAACTACCT |
| Suggested primer 2: | GTTCACGGGTATCCTGCTGT |