| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.044546 |
| Chromosome: | chromosome 2 |
| Location: | 4562334 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g100850 | (1 of 1) IPR000104//IPR005828//IPR011701//IPR020846 - Antifreeze protein, type I // Major facilitator, sugar transporter-like // Major facilitator superfamily // Major facilitator superfamily domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGCCCCTCACCGGCTCTCGTACCCTACGT |
| Internal bar code: | CCTGGCGTATGTTTGATACTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 66 |
| LEAP-Seq percent confirming: | 99.3289 |
| LEAP-Seq n confirming: | 148 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGAGGAGAGGGAGACAGG |
| Suggested primer 2: | TTGTAAGCGTGTGTGGGTGT |