Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.044564 |
Chromosome: | chromosome_3 |
Location: | 4058837 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre03.g172550 | PRMT1,PRM1 | Protein-/Histone-arginine N-methyltransferase | antisense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | AATGTCCTAGTACGCCCCCTGACTTTGAAT |
Internal bar code: | GTCAGGGCCCGTGCGGCGCAAG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 953 |
LEAP-Seq percent confirming: | 99.414 |
LEAP-Seq n confirming: | 3393 |
LEAP-Seq n nonconfirming: | 20 |
LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CAATGAAGAACGTGGTGGTG |
Suggested primer 2: | AAGAACACCCAGCAATACCG |