| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.044577 |
| Chromosome: | chromosome_12 |
| Location: | 736862 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre12.g495250 | FAL15 | Similar to Flagellar Associated Protein FAP31 | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TCGCGGCCACAGCTCTTCCCAACCCAGCGC |
| Internal bar code: | TGTGTGGCAACTGTTTGGGTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 47 |
| LEAP-Seq percent confirming: | 94.4724 |
| LEAP-Seq n confirming: | 188 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGAACTCTTACGCCACCGT |
| Suggested primer 2: | GATCACGGGAAGTCTGGTGT |