| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.044598 |
| Chromosome: | chromosome 7 |
| Location: | 3716905 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g337700 | PDC1 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCGCACCGCCCGGCCGCTGGTTGCGCTGC |
| Internal bar code: | AGCCGAAGCGTATAGGTAGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 171 |
| LEAP-Seq percent confirming: | 85.4442 |
| LEAP-Seq n confirming: | 452 |
| LEAP-Seq n nonconfirming: | 77 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGGCAGTTGTCAGTTGAAG |
| Suggested primer 2: | TAACTCACCAGCTGCCACAG |