| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.044608 |
| Chromosome: | chromosome 10 |
| Location: | 3675282 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g445900 | (1 of 1) PF00226//PF14901 - DnaJ domain (DnaJ) // Cleavage inducing molecular chaperone (Jiv90) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTACACCTGATGCTGCACCGCGCAGGCTGC |
| Internal bar code: | AATGGCCTTGGAGTCCTGTGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 503 |
| LEAP-Seq percent confirming: | 98.9011 |
| LEAP-Seq n confirming: | 4320 |
| LEAP-Seq n nonconfirming: | 48 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCATTTCGAAGCTGGAGAAC |
| Suggested primer 2: | CAGCGTGGTATCTGGACTCA |