Insertion junction: LMJ.RY0402.044619_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.044619
Chromosome:chromosome 3
Location:3183262
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre03.g165250 (1 of 1) PF01248//PF12874 - Ribosomal protein L7Ae/L30e/S12e/Gadd45 family (Ribosomal_L7Ae) // Zinc-finger of C2H2 type (zf-met) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCCGGAACGCCGGCACCCACCCACTCCGA
Internal bar code:CGAACTGTTCCTCTTTGCGACA

Confirmation - LEAP-Seq

LEAP-Seq distance:102
LEAP-Seq percent confirming:99.7275
LEAP-Seq n confirming:366
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:AACAACAACGAATCGGAAGG
Suggested primer 2:CTTCCCCTCTGAACCTCCTG