| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.044622 |
| Chromosome: | chromosome 16 |
| Location: | 4870162 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g685950 | (1 of 66) 2.7.12.1 - Dual-specificity kinase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCCGCGAGGTCGCCGCTGCCAGCGACAG |
| Internal bar code: | TCTAGGCTGGAGAGAGAAGGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 518 |
| LEAP-Seq percent confirming: | 99.523 |
| LEAP-Seq n confirming: | 6676 |
| LEAP-Seq n nonconfirming: | 32 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCACAACAACGACAACAAC |
| Suggested primer 2: | GGACAGGAGGAGGGTTCTTC |