| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.044639 |
| Chromosome: | chromosome 12 |
| Location: | 4968950 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g525850 | 3'UTR|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGTGTCATTGTGATGGGCGTTTGCGCTGG |
| Internal bar code: | TCGGATGCCGCAGCCGGCAGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 600 |
| LEAP-Seq percent confirming: | 99.6832 |
| LEAP-Seq n confirming: | 28632 |
| LEAP-Seq n nonconfirming: | 91 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGTCCCGCTATGTCTGTAT |
| Suggested primer 2: | TCTGTAACGTATTGCGGCTG |