| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.044656 |
| Chromosome: | chromosome 3 |
| Location: | 727507 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g146087 | (1 of 3) 1.14.11.33 - DNA oxidative demethylase / Alkylated DNA repair protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGCACCAACTACACGGCCACGCACAGGCG |
| Internal bar code: | TGAAAAAGAGCGCTTTTTTCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 572 |
| LEAP-Seq percent confirming: | 99.7241 |
| LEAP-Seq n confirming: | 1446 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCACGTGCAAATCAACAC |
| Suggested primer 2: | GTTCGTGGGTGGAGAGTGTT |