| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.044662 |
| Chromosome: | chromosome 9 |
| Location: | 7208871 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g412350 | (1 of 1) PTHR16557:SF4 - 2-OXOGLUTARATE-DEPENDENT DIOXYGENASE FAMILY PROTEIN-RELATED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCGCCTCCGCATCCCGGCCCCGCACACAA |
| Internal bar code: | ACCCTCTGTAGCCCACTGCCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 131 |
| LEAP-Seq percent confirming: | 98.2906 |
| LEAP-Seq n confirming: | 575 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGCAGTAGCAGTAGCGGCA |
| Suggested primer 2: | GATGTCGTTGGTGACATTGC |