| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.044669 |
| Chromosome: | chromosome 11 |
| Location: | 517225 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467598 | (1 of 1) IPR000104//IPR013763 - Antifreeze protein, type I // Cyclin-like | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCCTACATTATTGTTCTGGAAGTGGGAAG |
| Internal bar code: | ATCGGGATTCTGGAGCCGTTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 624 |
| LEAP-Seq percent confirming: | 97.1855 |
| LEAP-Seq n confirming: | 2797 |
| LEAP-Seq n nonconfirming: | 81 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACCAACTTTCAAACCGCAC |
| Suggested primer 2: | TGATGCATTTGCATTCCCTA |