| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.044698 |
| Chromosome: | chromosome 12 |
| Location: | 9423328 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g539900 | (1 of 1) K00166 - 2-oxoisovalerate dehydrogenase E1 component alpha subunit (BCKDHA, bkdA1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTTACATCACACATGACAGTTTATAAGGC |
| Internal bar code: | ATCGGTAACGTTTGGCCGCGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 339 |
| LEAP-Seq percent confirming: | 88.3562 |
| LEAP-Seq n confirming: | 1161 |
| LEAP-Seq n nonconfirming: | 153 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCGCTCGTTTTACCGTAG |
| Suggested primer 2: | AGGATACTTGTCGGTGGACG |