| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.044723 |
| Chromosome: | chromosome 13 |
| Location: | 1129850 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g569550 | (1 of 17) PF08016 - Polycystin cation channel (PKD_channel) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGGGGTGCTGTGGTGGCGGCACGGCGGCG |
| Internal bar code: | CAGGGTATCCTTCAGAACCCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 951 |
| LEAP-Seq percent confirming: | 99.8278 |
| LEAP-Seq n confirming: | 2319 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGGATTTGTGTATGTGTGC |
| Suggested primer 2: | GCCGCATTTTATGGTCTGTT |