Insertion junction: LMJ.RY0402.044734_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.044734
Chromosome:chromosome 4
Location:340731
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre04.g217908 FAP79 Ankyrin Repeat Flagellar Associated Protein 79; (1 of 1) PTHR24133//PTHR24133:SF21//PTHR24198 - FAMILY NOT NAMED // ANKYRIN REPEAT-CONTAINING PROTEIN // ANKYRIN REPEAT AND PROTEIN KINASE DOMAIN-CONTAINING PROTEIN 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGAATGCAACGCGCGTGAATGCCATCGCTT
Internal bar code:CCAGGCAAGTAAGGTCGGGGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:385
LEAP-Seq percent confirming:96.3783
LEAP-Seq n confirming:479
LEAP-Seq n nonconfirming:18
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:CGCTACCCTAGAAACGCTTG
Suggested primer 2:GAGGCTGGCTCTGATACTGG