Insertion junction: LMJ.RY0402.044745_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.044745
Chromosome:chromosome 8
Location:1657044
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre08.g365632 PPP32 Phosphoprotein phosphatase 2C-related; (1 of 70) 3.1.3.16 - Protein-serine/threonine phosphatase / Serine/threonine specific protein phosphatase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGAGGGGGGAGGGGGTTAACGTTGAACGAA
Internal bar code:CTCATAGCGCTTCTACATTGAG

Confirmation - LEAP-Seq

LEAP-Seq distance:29
LEAP-Seq percent confirming:97.6
LEAP-Seq n confirming:122
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:AGCTTAGAGCTGGAGTCCCC
Suggested primer 2:TTGAGAGAGGGTGAGGAGGA