| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.044752 |
| Chromosome: | chromosome 15 |
| Location: | 732201 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g640250 | (1 of 1) K07562 - nonsense-mediated mRNA decay protein 3 (NMD3) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATGATTATGTAAGGAGGGGGAGGGAGGGG |
| Internal bar code: | TCCCCGGCCCGGTCCCCAAAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 754 |
| LEAP-Seq percent confirming: | 99.6487 |
| LEAP-Seq n confirming: | 851 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGAAGGAGGAGAATCCTTG |
| Suggested primer 2: | GTGTTGTGCGTACCGATGAC |