Insertion junction: LMJ.RY0402.044754_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.044754
Chromosome:chromosome 2
Location:1025985
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre02.g080500 (1 of 5) 2.4.1.17 - Glucuronosyltransferase / Uridine diphosphoglucuronyltransferase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCTGGTCTTTCCCGGCGTCCACCGTGAGC
Internal bar code:TACTCTCGGTGGGTAGGTCACT

Confirmation - LEAP-Seq

LEAP-Seq distance:321
LEAP-Seq percent confirming:99.5821
LEAP-Seq n confirming:4528
LEAP-Seq n nonconfirming:19
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:TTAAACTGGGTTTTGGTCCG
Suggested primer 2:TGACCACAGGGAAAACATCA