Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.044776 |
Chromosome: | chromosome 5 |
Location: | 1501304 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre05.g243802 | (1 of 11) PF07173 - Protein of unknown function (DUF1399) (DUF1399) | CDS |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGTGCTTCAGTGCTGACGGGCTGGTGGTA |
Internal bar code: | GAACGACGAAGGGACGAAGACG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 442 |
LEAP-Seq percent confirming: | 97.892 |
LEAP-Seq n confirming: | 5619 |
LEAP-Seq n nonconfirming: | 121 |
LEAP-Seq n unique pos: | 50 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TCCTGGATTCTTTTGGATGC |
Suggested primer 2: | CACCCCCAGCATCTCATAGT |