Insertion junction: LMJ.RY0402.044776_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.044776
Chromosome:chromosome 5
Location:1501304
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre05.g243802 (1 of 11) PF07173 - Protein of unknown function (DUF1399) (DUF1399) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGTGCTTCAGTGCTGACGGGCTGGTGGTA
Internal bar code:GAACGACGAAGGGACGAAGACG

Confirmation - LEAP-Seq

LEAP-Seq distance:442
LEAP-Seq percent confirming:97.892
LEAP-Seq n confirming:5619
LEAP-Seq n nonconfirming:121
LEAP-Seq n unique pos:50

Suggested primers for confirmation by PCR

Suggested primer 1:TCCTGGATTCTTTTGGATGC
Suggested primer 2:CACCCCCAGCATCTCATAGT