| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.044784 |
| Chromosome: | chromosome 10 |
| Location: | 715461 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g422550 | (1 of 274) IPR020683 - Ankyrin repeat-containing domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGAGACATCCCGCCACTTCGCCGGGCGG |
| Internal bar code: | TGTTACGTGTCTCGGACAACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1008 |
| LEAP-Seq percent confirming: | 97.9791 |
| LEAP-Seq n confirming: | 1406 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGATCAGCAGACGTGGACTC |
| Suggested primer 2: | ATCCTTGCTCATCGAACCAC |