Insertion junction: LMJ.RY0402.044789_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.044789
Chromosome:chromosome 17
Location:6779833
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre17.g744797 (1 of 1) PF05623 - Protein of unknown function (DUF789) (DUF789) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGCGCCGCCGGTAGCGCCGCCACTGCCGC
Internal bar code:CATTATTAAGGCTCTGCCACG

Confirmation - LEAP-Seq

LEAP-Seq distance:1
LEAP-Seq percent confirming:99.8131
LEAP-Seq n confirming:1068
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CACCTTGCAAGACCAGCAG
Suggested primer 2:TGAACGATCGGTTGTTGAAG