| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.044793 |
| Chromosome: | chromosome 3 |
| Location: | 298239 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g144584 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGGGTGCGAGCACACACCTGCATGGCCTT |
| Internal bar code: | GTCGGCTAATTGCCTAAGCTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1120 |
| LEAP-Seq percent confirming: | 99.6851 |
| LEAP-Seq n confirming: | 10448 |
| LEAP-Seq n nonconfirming: | 33 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTTGTTTCCCGCTCCCTAT |
| Suggested primer 2: | CTGCTCCTGCTCTTGGACTC |