| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.044840 |
| Chromosome: | chromosome 7 |
| Location: | 112866 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g312950 | HEL32 | DEAD/DEAH box DNA/RNA helicase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGAGGTGTGGCGAAAGGCAGAGACTTGGC |
| Internal bar code: | GGTTGGCTTGTCCGCGACGTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 617 |
| LEAP-Seq percent confirming: | 99.6625 |
| LEAP-Seq n confirming: | 11220 |
| LEAP-Seq n nonconfirming: | 38 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGGTTAGGACCTTTCACGG |
| Suggested primer 2: | TGCCAACTACATCAAGCTGC |