| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.044853 |
| Chromosome: | chromosome 9 |
| Location: | 7198962 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g412250 | (1 of 781) IPR000104 - Antifreeze protein, type I | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCATCCCCACCACCCCACAGCTACTGCAG |
| Internal bar code: | GGGTGGCACCGCCCCTCTCTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 727 |
| LEAP-Seq percent confirming: | 74.6269 |
| LEAP-Seq n confirming: | 50 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAACGGGATTGTTGTCTGTG |
| Suggested primer 2: | AGGCAGTGAGTGGCAGAACT |