Insertion junction: LMJ.RY0402.044877_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.044877
Chromosome:chromosome 17
Location:2690938
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre17.g717850 PHC8 (1 of 1) IPR003882//IPR024616//IPR027417 - Pistil-specific extensin-like protein // Pherophorin // P-loop containing nucleoside triphosphate hydrolase; Pherophorin-chlamydomonas homolog 8 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCTGACTCTTGGGTTTGTTCTTCATAGCTC
Internal bar code:AGCACCTACGATAACATGGTCT

Confirmation - LEAP-Seq

LEAP-Seq distance:1038
LEAP-Seq percent confirming:99.5397
LEAP-Seq n confirming:41307
LEAP-Seq n nonconfirming:191
LEAP-Seq n unique pos:49

Suggested primers for confirmation by PCR

Suggested primer 1:GGTTCTCACGAAGCAGGAAG
Suggested primer 2:GCAACAGAGCATGGAGACAA