| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.044891 |
| Chromosome: | chromosome 12 |
| Location: | 6259468 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g537000 | (1 of 1) PF02041 - Auxin binding protein (Auxin_BP) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAATGTTGTTACACGCGCGTACAAACAGC |
| Internal bar code: | CCCGAGGGAATACTTCAATCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 776 |
| LEAP-Seq percent confirming: | 99.7548 |
| LEAP-Seq n confirming: | 2848 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATACACGTACCGTGCGAACA |
| Suggested primer 2: | CAGAAAGCTCCTTACCGACG |