| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.044914 |
| Chromosome: | chromosome 4 |
| Location: | 2886197 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g224450 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATATCTCGTCCTTTGTATCGAGCCTTTTTC |
| Internal bar code: | GGGGGCAATTGAGTGGCGGGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 789 |
| LEAP-Seq percent confirming: | 98.5271 |
| LEAP-Seq n confirming: | 1271 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACGCTTGGATATCGGTCAG |
| Suggested primer 2: | TTCCGACGTAGACAGTGCAG |