| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.044983 |
| Chromosome: | chromosome 12 |
| Location: | 4534314 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g522100 | (1 of 10) PTHR10174 - RETINALDEHYDE BINDING PROTEIN-RELATED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGAGGACCGGAGGAGCTTGACTAGTCTG |
| Internal bar code: | ATGTCTTTTGAGGTCGTACGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 591 |
| LEAP-Seq percent confirming: | 98.894 |
| LEAP-Seq n confirming: | 3666 |
| LEAP-Seq n nonconfirming: | 41 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGTTTGCGTGCTTTCTATG |
| Suggested primer 2: | GCCTGGTCGACTTGCTTTAG |