| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.044996 |
| Chromosome: | chromosome 17 |
| Location: | 3529742 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g725250 | (1 of 39) IPR009003 - Peptidase S1, PA clan | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCACACTCGCAGCTCCCGCTTTCTTTCGC |
| Internal bar code: | AATATATGGTTAACGTAAATGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 178 |
| LEAP-Seq percent confirming: | 98.6842 |
| LEAP-Seq n confirming: | 75 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAATCTTCATGTGTGGGAA |
| Suggested primer 2: | GCAGAAGTGCATGACAAGGA |