| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.044998 |
| Chromosome: | chromosome 17 |
| Location: | 1547409 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g707400 | (1 of 1) PF00013//PF13014 - KH domain (KH_1) // KH domain (KH_3) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGATAGCGGGCTCAAGAAAGCGCCCGCTG |
| Internal bar code: | CGCTATCTAAGAGTCGGCGCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 680 |
| LEAP-Seq percent confirming: | 99.5026 |
| LEAP-Seq n confirming: | 3201 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGTTGAGTCCTGCTCAGCC |
| Suggested primer 2: | GCACATTGTCCATTCGTCAC |