Insertion junction: LMJ.RY0402.045034_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.045034
Chromosome:chromosome 16
Location:2076556
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre16.g657450 MPA12 Metallophosphoesterase/metallo-dependent phosphatase; (1 of 1) 3.6.1.13//3.6.1.16//3.6.1.53 - ADP-ribose diphosphatase / ADPR-PPase // CDP-glycerol diphosphatase / CDP-glycerol pyrophosphatase // Mn(2+)-dependent ADP-ribose/CDP-alcohol diphosphatase / Mn(2+)-dependent ADP-ribose/CDP-alcohol pyrophosphatase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCATCTTCCTGAATGGTGTGTGGCTGGTGG
Internal bar code:CTGGAATTACGTGCCGGTTCGC

Confirmation - LEAP-Seq

LEAP-Seq distance:724
LEAP-Seq percent confirming:92.4016
LEAP-Seq n confirming:8476
LEAP-Seq n nonconfirming:697
LEAP-Seq n unique pos:38

Suggested primers for confirmation by PCR

Suggested primer 1:TGCAGGGTTAGAGGAGGCTA
Suggested primer 2:GCGGGTAGATAATGCGTGTT