Insertion junction: LMJ.RY0402.045035_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.045035
Chromosome:chromosome 16
Location:3583326
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre16.g682013 (1 of 2) PTHR30545:SF2 - SUGAR FERMENTATION STIMULATION PROTEIN A intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATTATTTCGTATCGTACAGTTGGTGGAGGA
Internal bar code:TAATCGACATCGTCATGACCTG

Confirmation - LEAP-Seq

LEAP-Seq distance:923
LEAP-Seq percent confirming:89.3443
LEAP-Seq n confirming:1417
LEAP-Seq n nonconfirming:169
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:TGACACCACACCTCACCCTA
Suggested primer 2:ATTTCGTGTGCATGTGTCGT