| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.045035 |
| Chromosome: | chromosome 16 |
| Location: | 3583327 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g682013 | (1 of 2) PTHR30545:SF2 - SUGAR FERMENTATION STIMULATION PROTEIN A | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGTAGGGCTGGGTGGTTAGGTTGGTGGTG |
| Internal bar code: | AATTCGTATCACTGGATGTTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 621 |
| LEAP-Seq percent confirming: | 97.2992 |
| LEAP-Seq n confirming: | 9583 |
| LEAP-Seq n nonconfirming: | 266 |
| LEAP-Seq n unique pos: | 43 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGACACCACACCTCACCCTA |
| Suggested primer 2: | ATTTCGTGTGCATGTGTCGT |