| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.045040 |
| Chromosome: | chromosome 12 |
| Location: | 7690507 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g554150 | (1 of 18) PF12906 - RING-variant domain (RINGv) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTCGTGTCAGGGCGGTTGCTTGCTCGTAT |
| Internal bar code: | TCCAGCTCGCCCCTTCCGTTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 615 |
| LEAP-Seq percent confirming: | 88.8889 |
| LEAP-Seq n confirming: | 16 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCACACAGGGGTCGTATCTT |
| Suggested primer 2: | GGATCAACACCAGGAGTCGT |