Insertion junction: LMJ.RY0402.045040_4

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.045040
Chromosome:chromosome 13
Location:3639920
Confidence (%):73
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCGCGATGACGCTCGAGGCGCCTAAAACAA
Internal bar code:GGGCCGCGGTACTCAACACCCA

Confirmation - LEAP-Seq

LEAP-Seq distance:531
LEAP-Seq percent confirming:99.5876
LEAP-Seq n confirming:483
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:TGTTTATGCCACCCTTGTGA
Suggested primer 2:CTCCTGCTGAAGCTAATGGG