Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.045042 |
Chromosome: | chromosome 6 |
Location: | 7032300 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre06.g296900 | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTGATGTGACTGCGGCCAGCATCCTCTGC |
Internal bar code: | CGACCTAGACTTTTCAACGCCC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 391 |
LEAP-Seq percent confirming: | 99.3219 |
LEAP-Seq n confirming: | 14355 |
LEAP-Seq n nonconfirming: | 98 |
LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AGCTCGTAGCCAATGACGTT |
Suggested primer 2: | ACGTTCATTTTGTTCTCGGC |