| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.045046 |
| Chromosome: | chromosome 1 |
| Location: | 2257315 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g012350 | (1 of 1) K02331 - DNA polymerase phi [EC:2.7.7.7] (POL5, MYBBP1A) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGAGCAGGATGGAGGGAGGACTGCGTAGA |
| Internal bar code: | ATGTTATGGCCACGGATAGGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 935 |
| LEAP-Seq percent confirming: | 99.1986 |
| LEAP-Seq n confirming: | 5818 |
| LEAP-Seq n nonconfirming: | 47 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGGGGAGTGGTAGAGAGTG |
| Suggested primer 2: | CAGAAGGGCAAGGAGAAGG |