Insertion junction: LMJ.RY0402.045071_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.045071
Chromosome:chromosome 6
Location:5655880
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g286200 (1 of 4) PTHR22911//PTHR22911:SF6 - ACYL-MALONYL CONDENSING ENZYME-RELATED // SOLUTE CARRIER FAMILY 35 MEMBER G1 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATGATACCAGGTGATGCTTTGAGTTTGGAA
Internal bar code:CTCTCAGCTTGTAAGTCATCAA

Confirmation - LEAP-Seq

LEAP-Seq distance:812
LEAP-Seq percent confirming:79.2804
LEAP-Seq n confirming:3658
LEAP-Seq n nonconfirming:956
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:TACCAACCGCCATCACAGTA
Suggested primer 2:AGGGAACTGGCATGTGTTTC