| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.045084 |
| Chromosome: | chromosome 1 |
| Location: | 3874238 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g025150 | CPLD15 | conserved organelle protein with lipase active site; (1 of 1) PTHR11440:SF48 - HYDROLASE-LIKE PROTEIN | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAATTCACAAGCAGTGTCTGAATGTTGCGT |
| Internal bar code: | ATTACCTGCGTGTCTCGTTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 655 |
| LEAP-Seq percent confirming: | 99.5635 |
| LEAP-Seq n confirming: | 12089 |
| LEAP-Seq n nonconfirming: | 53 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGAAACTGTGAAGCTGTGC |
| Suggested primer 2: | GGCACTACAACACAGAGGCA |