Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.045116 |
Chromosome: | chromosome_2 |
Location: | 6181959 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre02.g113751 | sense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | ACACCCGGTCGCACAGGGGGTCATTCACGT |
Internal bar code: | TTGTACGACTATTTTGTAGTTT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 497 |
LEAP-Seq percent confirming: | 0.815788 |
LEAP-Seq n confirming: | 136 |
LEAP-Seq n nonconfirming: | 16535 |
LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CCATTCGGTCGTACTCACCT |
Suggested primer 2: | AGTGCCGAAAGGAACCCTAT |