| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.045118 |
| Chromosome: | chromosome 17 |
| Location: | 6232772 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g742200 | (1 of 1) PTHR22870:SF220 - RCC1 AND BTB DOMAIN-CONTAINING PROTEIN 2 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGTGAAGCCGATGCCGCACGCCACCTGGT |
| Internal bar code: | CGTCTCGCCTCCCACGGCGAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 794 |
| LEAP-Seq percent confirming: | 99.6757 |
| LEAP-Seq n confirming: | 7070 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGCTCAAGGTCAACGGAGA |
| Suggested primer 2: | TAGCTGCATGCAATCTACCG |