| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.045124 |
| Chromosome: | chromosome 6 |
| Location: | 6473361 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g292950 | (1 of 1) K03505 - DNA polymerase delta subunit 4 (POLD4) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTTCAGCCCGCGCCCAGAAATACATGACA |
| Internal bar code: | TGATGTAAAAGTTTTCGGGTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 236 |
| LEAP-Seq percent confirming: | 98.8825 |
| LEAP-Seq n confirming: | 4159 |
| LEAP-Seq n nonconfirming: | 47 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTTGCGCTTAAAAGGCTGT |
| Suggested primer 2: | CCTGCTAGGTCCTTCGTCAG |