| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.045124 |
| Chromosome: | chromosome 6 |
| Location: | 6473408 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g292950 | (1 of 1) K03505 - DNA polymerase delta subunit 4 (POLD4) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCAGTAGCGGCACTTCCGGGCGCCCCTTT |
| Internal bar code: | GGTTGCGGGTAGAAGTGCAGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 916 |
| LEAP-Seq percent confirming: | 98.6704 |
| LEAP-Seq n confirming: | 6976 |
| LEAP-Seq n nonconfirming: | 94 |
| LEAP-Seq n unique pos: | 34 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTTGCGCTTAAAAGGCTGT |
| Suggested primer 2: | CCTGCTAGGTCCTTCGTCAG |