Insertion junction: LMJ.RY0402.045136_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.045136
Chromosome:chromosome 8
Location:1061344
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre08.g362150 FAP361,FAP154 Flagellar Associated Protein 361 with PAS Sensory Domain; (1 of 28) PTHR31600//PTHR31600:SF2 - FAMILY NOT NAMED // TINY MACROCYSTS PROTEIN B-RELATED CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGAATAAACCGCTGCTGGCCGGGGCGGTG
Internal bar code:GTGCTTCCCGCCCTCCCACGAA

Confirmation - LEAP-Seq

LEAP-Seq distance:864
LEAP-Seq percent confirming:92.8571
LEAP-Seq n confirming:13
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CCCATGTTCGTTACATGCTG
Suggested primer 2:ATATACATGAGCGGATCGCC