| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.045136 |
| Chromosome: | chromosome 10 |
| Location: | 5101754 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g456150 | HLD3 | (1 of 1) PTHR10992:SF701 - SERINE HYDROLASE-RELATED; Haloalkane dehalogenase-like hydrolase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGACAACACCACATCGCCCCTCGCACACCG |
| Internal bar code: | ATGGAAGGGAGTCATCTTGATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 971 |
| LEAP-Seq percent confirming: | 93.1719 |
| LEAP-Seq n confirming: | 13509 |
| LEAP-Seq n nonconfirming: | 990 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCGTCGTTTGTATGGTATG |
| Suggested primer 2: | GCCTTGTCCTCTGTCTCGTC |