| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.045142 |
| Chromosome: | chromosome 6 |
| Location: | 8720406 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g309800 | MOT57,MOT2 | Predicted protein in motile organisms; (1 of 18) PTHR19862:SF14 - WD REPEAT-CONTAINING PROTEIN 48 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGACATGACATGACGTGCTGCGTGCGATG |
| Internal bar code: | TTACACGGGGTAGTGGGTTGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 817 |
| LEAP-Seq percent confirming: | 99.2669 |
| LEAP-Seq n confirming: | 33852 |
| LEAP-Seq n nonconfirming: | 250 |
| LEAP-Seq n unique pos: | 72 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGTGCTCTCATGCATGCTC |
| Suggested primer 2: | AAGCTGCTGCCTGCATTATT |