Insertion junction: LMJ.RY0402.045197_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.045197
Chromosome:chromosome 1
Location:4167120
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre01.g027500 (1 of 2) IPR001870//IPR003877//IPR013320 - B30.2/SPRY domain // SPRY domain // Concanavalin A-like lectin/glucanase domain 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAGCAGGGCGCACGCCACGCTTCCCCACCC
Internal bar code:AAAGAAGAAGGTCCGCGACGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:1071
LEAP-Seq percent confirming:99.0128
LEAP-Seq n confirming:2006
LEAP-Seq n nonconfirming:20
LEAP-Seq n unique pos:22

Suggested primers for confirmation by PCR

Suggested primer 1:ACTTTTGATACACTGCCGGG
Suggested primer 2:AAATGTTTGCGGACTTACGG