| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.045201 |
| Chromosome: | chromosome 12 |
| Location: | 8309368 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g549850 | (1 of 1) PF09445 - RNA cap guanine-N2 methyltransferase (Methyltransf_15) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCACCAGCACCATCAGCTTGTTCTTCATC |
| Internal bar code: | TAGGTTCAATGAGTGAGTGTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 213 |
| LEAP-Seq percent confirming: | 99.0674 |
| LEAP-Seq n confirming: | 956 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGCCAGACCCCATTACAGT |
| Suggested primer 2: | TAGTTGGGGTGTCGTGTTCA |