| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.045235 |
| Chromosome: | chromosome 8 |
| Location: | 3341375 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g374100 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTTGGTGCAGCCTGTGGCTTAGGCTGTTG |
| Internal bar code: | ACAGATAGTTGCCAAGGCCGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 975 |
| LEAP-Seq percent confirming: | 91.2095 |
| LEAP-Seq n confirming: | 7066 |
| LEAP-Seq n nonconfirming: | 681 |
| LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGGTTGCAAATATGTGAGG |
| Suggested primer 2: | CTTCAGTGGGAGGACTCAGC |