| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.045263 |
| Chromosome: | chromosome 4 |
| Location: | 694472 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g217954 | (1 of 1) K05850 - Ca2+ transporting ATPase, plasma membrane (ATP2B) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCGAACCCAACCAAACAGGTGCTGCGCAA |
| Internal bar code: | TATAACTGACGACCCTACCTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 434 |
| LEAP-Seq percent confirming: | 99.445 |
| LEAP-Seq n confirming: | 1075 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAATGGTGCTTGATGGGACA |
| Suggested primer 2: | GATCCAGCCTGTGGAAGTGT |